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Forum: Bioinformatics 03-13-2017, 01:12 PM
Replies: 8
Views: 1,236
Posted By sindrle
Thats great! Any input on how to normalise...

Thats great!

Any input on how to normalise the counts? i.e. "counts/library size"?
Forum: Bioinformatics 03-13-2017, 12:55 PM
Replies: 8
Views: 1,236
Posted By sindrle
Thank you very much! The two transcripts...

Thank you very much!

The two transcripts each have 2 unique sections (exon-a1 for one, and exon-1b for the other). I guess I can just define where these sequences are, in a GTF-file?, and use...
Forum: Bioinformatics 03-13-2017, 09:03 AM
Replies: 8
Views: 1,236
Posted By sindrle
Ok, so I got the transcript-sequences for the two...

Ok, so I got the transcript-sequences for the two transcripts of interest. Can you please explain how I should use them as reference for Bowtie2 (file format etc.)? An example with code would be very...
Forum: Bioinformatics 03-12-2017, 06:57 AM
Replies: 8
Views: 1,236
Posted By sindrle
Thats interesting! Yes, I have the full...

Thats interesting!

Yes, I have the full transcript sequences. What do you think is the pros and cons between these approaches?
Forum: Bioinformatics 03-11-2017, 07:36 AM
Replies: 8
Views: 1,236
Posted By sindrle
Analysing only exons from ONE gene using RNAseq

I know that DEXseq and/or Cuffdiff etc. may be used of exon-levels analyses.

However, I am only interested in ONE gene, because a novel exon is discovered in this gene, by other researches. There...
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