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Forum: Bioinformatics 11-24-2018, 01:24 AM
Replies: 0
Views: 790
Posted By Moip
Umi-tools

Hello,
i'am processing Fastq with umis, when i use umi-tools count, (after umi extraction, mapping and deduplication) the output file is emty. i am wondering if anyone can help me out ?
Thank you
Forum: Bioinformatics 09-27-2018, 11:12 PM
Replies: 6
Views: 1,083
Posted By Moip
Thank you, i ll give a try. Do i ignore UMi for...

Thank you, i ll give a try. Do i ignore UMi for this analysis?
Forum: Bioinformatics 09-27-2018, 06:48 AM
Replies: 6
Views: 1,083
Posted By Moip
Thank you for you reply. i need to understand...

Thank you for you reply.
i need to understand some details, once i have a BAM file, before variant calling i have to remove duplicate, recalibrate BAM? run other metrics ?
sorry i am completely new...
Forum: Bioinformatics 09-26-2018, 11:54 PM
Replies: 6
Views: 1,083
Posted By Moip
I have three normal samples and looking for...

I have three normal samples and looking for germline characteristics.
Mapping with BWA-MEM.
Thank you for you help Richard
Forum: Bioinformatics 09-26-2018, 10:13 AM
Replies: 6
Views: 1,083
Posted By Moip
metrics on BAM file

Hi everyone,
I am doing exome sequencing, paired end, but not used to analyze reads.
After alignement, how do i proceed the BAM file?
Thank you
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