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Forum: 454 Pyrosequencing 04-17-2012, 08:05 AM
Replies: 17
Views: 4,089
Posted By JKistler
Hi Anthony, Well done on that! May I ask,...

Hi Anthony,

Well done on that! May I ask, what method you used to purify your 16S amplicons/eliminate primer dimers? Also, how many copies per bead did you aim for & what % enrichment did you get...
Forum: 454 Pyrosequencing 04-10-2012, 06:11 AM
Replies: 7
Views: 4,673
Posted By JKistler
Thanks to all for your advice. I'll go with a gel...

Thanks to all for your advice. I'll go with a gel cut after pooling and optimise PCR as best as possible to reduce primer dimers. Any improvement, would be good. That's worrying to hear that the...
Forum: 454 Pyrosequencing 04-05-2012, 02:56 AM
Replies: 7
Views: 4,673
Posted By JKistler
Amplicon sequencing - gel cut purification

Hi all,

For our next 454 amplicon sequencing run we are thinking to do a gel cut purification of our amplicons in order to eliminate small fragments e.g primer dimers (they may be getting through...
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