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Forum: Illumina/Solexa 07-08-2020, 02:05 PM
Replies: 1
Views: 2,830
Posted By Lechu
Unhappy Demultiplexing failures in MiSeq run

Hi All!

I am sequencing (on a MySeq platform) a custom library containing 4 i7 indexes. In principle, it works, but when I look on demultiplexing stats, there is quite a large proportion of...
Forum: Bioinformatics 06-17-2019, 10:59 AM
Replies: 6
Views: 2,180
Posted By Lechu
This is exactly how they are in the reference,...

This is exactly how they are in the reference, silly me. But is there some hidden method in this...?

$ grep "^>" GRCm38.primary_assembly.genome.fa | head -20
>chr1 1
>chr2 2
>chr3 3
>chr4 4...
Forum: Bioinformatics 06-17-2019, 10:38 AM
Replies: 6
Views: 2,180
Posted By Lechu
Question BBmap generates Bam files with duplications in sequence sequence names

After running BBmap with the command like this:
java -ea -Xmx30g align2.BBMap build=1 overwrite=true fastareadlen=500 -Xmx30g in=8601_RG15-S1_IndexD706-D508_GAATTCGT+GTACTGAC_L007_R1_001.fastq.gz...
Forum: General 02-13-2019, 08:59 AM
Replies: 1
Views: 3,090
Posted By Lechu
barcoded RNA adapter ligation for multiplexing

Hi All,

This is my first post here, so hello everyone! :)

I'm wondering if it is possible to use RNA adapter ligated to (preferably) 3' end of the RNA for multiplexing the samples prior to RNA...
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