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Forum: Bioinformatics 11-16-2017, 11:38 AM
Replies: 0
Views: 1,020
Posted By antoinefelden
RNA-seq, GO enrichment: how does GOseq package deals with missing annotations

Hi,

I'm working on a RNA-seq experiment of a non-model organism. I assembled a transcriptome using Trinity, and ran GOseq to examine for GO enrichment in DE transcript clusters. I use as...
Forum: Sample Prep / Library Generation 05-14-2017, 02:29 PM
Replies: 1
Views: 736
Posted By antoinefelden
Fast migration in RNA Bioanalyzer assay

My latest RNA Bioanalyzer runs produced unexpected results, with migration time being reduced for both the ladder and the samples, see attached profiles for comparison (below in this order: abnormal...
Forum: Bioinformatics 12-09-2016, 02:03 PM
Replies: 10
Views: 4,155
Posted By antoinefelden
Hi Brian, Would you say that the problem...

Hi Brian,

Would you say that the problem comes from the missing output option?
If so, do you know how to fix that error?

If that helps, it may also be related to the samtools version, I'm...
Forum: Bioinformatics 12-08-2016, 03:26 PM
Replies: 10
Views: 4,155
Posted By antoinefelden
I have the same problem, looking at the SAMTools...

I have the same problem, looking at the SAMTools manual, I have the feeling there is a problem on the output option of this command:



Could it be missing a "-o" before the filename? Have a look...
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