Forum: Sample Prep / Library Generation
09-10-2019, 02:15 AM
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Replies: 5
Views: 1,956
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Forum: Illumina/Solexa
03-13-2018, 02:15 AM
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Replies: 1
Views: 1,092
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Forum: Illumina/Solexa
02-20-2018, 05:02 AM
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Replies: 0
Views: 1,423
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Forum: Sample Prep / Library Generation
11-03-2017, 01:59 AM
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Replies: 2
Views: 2,077
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Forum: Sample Prep / Library Generation
10-09-2017, 05:27 AM
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Replies: 7
Views: 6,743
The kit includes so-called "SPB" (Sample...
The kit includes so-called "SPB" (Sample purification Beads) so you don't need AMpure XP anymore (except for an initial hypothetic sample purification).
It might not justify completely the higher...
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Forum: General
09-27-2017, 05:55 AM
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Replies: 1
Views: 1,836
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Forum: Illumina/Solexa
09-19-2017, 05:44 AM
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Replies: 5
Views: 1,721
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Forum: Illumina/Solexa
09-14-2017, 06:35 AM
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Replies: 5
Views: 1,721
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Forum: Sample Prep / Library Generation
09-14-2017, 06:28 AM
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Replies: 1
Views: 1,149
Hello,
In my opinion, the most sensitive...
Hello,
In my opinion, the most sensitive reagent in the kit is the ligation mix.
But it's hard to say if it suffered from standing at RT without testing it.
if you have any doubt, you can try to...
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Forum: Sample Prep / Library Generation
07-19-2017, 11:34 PM
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Replies: 2
Views: 1,131
Hello,
This kind of protocol is typical of...
Hello,
This kind of protocol is typical of smallRNAseq.
It uses a truncated T4 RNA ligase 2 and a modified 3' adapter.
Trunc. T4 RNA ligase is unable to adenylate the 5' end of its substrate so...
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Forum: Illumina/Solexa
07-19-2017, 04:42 AM
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Replies: 6
Views: 1,874
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Forum: Illumina/Solexa
07-18-2017, 02:02 AM
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Replies: 6
Views: 1,874
You bought a HS2500? I thought the machine was...
You bought a HS2500? I thought the machine was being discontinued by Illumina.
Don't get me wrong: the machine itself is awesome, very flexible with high quality runs. We are using one for more than...
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Forum: Sample Prep / Library Generation
07-10-2017, 04:46 AM
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Replies: 5
Views: 3,127
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Forum: Sample Prep / Library Generation
06-15-2017, 12:02 AM
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Replies: 1
Views: 995
Hello,
Did you only use the nanodrop to...
Hello,
Did you only use the nanodrop to quantify your RNA samples or did you also perform some integrity checking on bioanalyzer?
Your concentration is pretty low and your nanodrop ratios are not...
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Forum: RNA Sequencing
03-17-2017, 02:51 AM
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Replies: 7
Views: 2,107
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Forum: Sample Prep / Library Generation
02-10-2017, 06:36 AM
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Replies: 8
Views: 2,225
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Forum: Sample Prep / Library Generation
02-07-2017, 07:48 AM
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Replies: 0
Views: 992
How to interprete Stacks partial results?
Hi,
I'm posting here but I'm not sure I'm in the correct section of the forum. If so, I apologize in advance.
So, we are performing RADseq experiments.
DNA comes from differents organisms and...
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Forum: Sample Prep / Library Generation
02-06-2017, 04:43 AM
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Replies: 4
Views: 3,534
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Forum: Sample Prep / Library Generation
01-19-2017, 02:34 AM
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Replies: 10
Views: 3,239
It's only an issue if you're size selecting...
It's only an issue if you're size selecting before the PCR. At least, that's what we figured out from our experiments.
If you perform the PCR before the size selection, even if you loose material...
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Forum: Sample Prep / Library Generation
01-18-2017, 07:40 AM
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Replies: 1
Views: 1,558
Judging by the picture you joined to your...
Judging by the picture you joined to your message, they are added during the PCR step.
Indexing during PCR is rather common ine Illumina's protocols especially when for amplicons and when using...
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Forum: Sample Prep / Library Generation
01-18-2017, 07:27 AM
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Replies: 10
Views: 3,239
As sonication is performed before Immuno...
As sonication is performed before Immuno precipitation, you should be able to see your sonication profile on a bioanalyzer, especially when starting from 50 millions cells.
I advise to quantify you...
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Forum: Sample Prep / Library Generation
11-17-2016, 05:19 AM
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Replies: 4
Views: 2,146
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Forum: Sample Prep / Library Generation
11-16-2016, 08:33 AM
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Replies: 4
Views: 2,146
Hello,
Usually, we perform a AMPure XP...
Hello,
Usually, we perform a AMPure XP purification step after the P1 ligation and we do not face any issue with the sonication. Maybe you should give it a try...
H.
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Forum: Sample Prep / Library Generation
10-17-2016, 07:38 AM
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Replies: 2
Views: 2,045
Hi Irene,
First of all, I don't have experiment...
Hi Irene,
First of all, I don't have experiment with the nextseq system. We have a Hiseq2500 but I suppose the issue and solutions are the same.
What we usually do when our library concentration is...
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Forum: Illumina/Solexa
10-05-2016, 06:11 AM
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Replies: 19
Views: 8,897
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