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Forum: Sample Prep / Library Generation 12-22-2019, 08:50 AM
Replies: 20
Views: 13,289
Posted By AmitChaurasia
This is truly helpful to solve my long pending...

This is truly helpful to solve my long pending case with the customer...

Kindest Regard's
Amit
Forum: Illumina/Solexa 11-28-2019, 07:04 PM
Replies: 2
Views: 1,035
Posted By AmitChaurasia
Hi Chenyudr, These libraries are PCR based...

Hi Chenyudr,

These libraries are PCR based target amplification but UMI based.
Forum: Illumina/Solexa 11-20-2019, 10:36 AM
Replies: 2
Views: 1,035
Posted By AmitChaurasia
Allele Frequency Reproducibility: An interesting observation

Hi All,

I have got an very interesting observation during troubleshooting of an experiment with patient sample as described below (I would like to have your opinion at each point before moving...
Forum: Metagenomics 04-17-2018, 02:23 AM
Replies: 13
Views: 5,631
Posted By AmitChaurasia
Thanks a lot Phillip for your valuable inputs. ...

Thanks a lot Phillip for your valuable inputs.

I have gone through your suggestions and looked into the data in greater details wrt possiblity of primer-dimer or adapter dimers. I have checked the...
Forum: Metagenomics 04-15-2018, 06:48 PM
Replies: 13
Views: 5,631
Posted By AmitChaurasia
Hi Kinman, Thanks for your reply, I have...

Hi Kinman,

Thanks for your reply, I have taken random samples to check with Bioanalyzer and no short peaks were found. Also fastq files were checked to have 301 base length only.

So this can...
Forum: Metagenomics 04-15-2018, 01:27 AM
Replies: 13
Views: 5,631
Posted By AmitChaurasia
I had a Metagenomic 16s rRNA run on MiSeq v3 kit 2 x300 cycles. with good PF >89% but

Hi All,

I had a metagenomic run on MiSeq using v3 kits of 16s rRNA v3-v4 region. I have loaded 8pM pooled library with 10% PhiX spike-in. MiSeq run had 89% PF with 16 M raw reads but with very low...
Forum: Bioinformatics 08-13-2016, 02:23 AM
Replies: 6
Views: 3,887
Posted By AmitChaurasia
Thank You Thias... I hope this should work. I...

Thank You Thias... I hope this should work. I believe that this could be an issue for different version of R.

Best,
Amit
Forum: Bioinformatics 08-08-2016, 01:50 AM
Replies: 6
Views: 3,887
Posted By AmitChaurasia
Hi here I am getting same error. geom_vline...

Hi here I am getting same error.

geom_vline requires the following missing aesthetics: xintercept

could anyone help... since I tried all aspects of installing reinstalling the things.

Thanks,
Forum: General 07-01-2016, 04:05 AM
Replies: 26
Views: 14,975
Posted By AmitChaurasia
Thanks Tony & Chipper for your beautiful...

Thanks Tony & Chipper for your beautiful expression... I was also curious about the question asked by chaomeizhang. Thanks chaomeizhang.
Forum: Bioinformatics 03-11-2013, 04:32 AM
Replies: 3
Views: 2,947
Posted By AmitChaurasia
Dear GenoMax, Thanks a lot for the reply,...

Dear GenoMax,

Thanks a lot for the reply, YEs our HiSeq got upgraded to 2500 from 2000 and hence the option of Zipped BCL file have been selected.

I have followed you instructions and the job...
Forum: Bioinformatics 03-09-2013, 11:56 PM
Replies: 3
Views: 2,947
Posted By AmitChaurasia
Unhappy Zipped BCL to FastQ

Hi Friends,

When I used CASAVA 1.8.2 to convert BCL to FastQ, it showd and error that *.bcl file not found. When I go to folder to check for the file i found *.bcl.gz file was present. In this way...
Forum: Sample Prep / Library Generation 03-04-2013, 10:59 PM
Replies: 3
Views: 978
Posted By AmitChaurasia
thanks dear Kmcarr and krobison for the reply, ...

thanks dear Kmcarr and krobison for the reply,

I am satisfied with the answers, but now more curious about how efficient the coverage would be and will it cost effective ?

thanks ?
Forum: Sample Prep / Library Generation 03-02-2013, 07:47 AM
Replies: 3
Views: 978
Posted By AmitChaurasia
Sequencing smaller regions in the genome ?

Hi Friends,

I am new to sequencing field. I have to sequence 10mb region from the human genome. I do not want to sequence whole genome. Kindly suggest me the ways to proceed.

Best,
Amit
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