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Forum: Illumina/Solexa 11-07-2017, 04:33 AM
Replies: 13
Views: 1,851
Posted By ATGCT
Hi, thanks :-) I will have a look at it. But the...

Hi, thanks :-) I will have a look at it. But the insert size should be around 500 bp - I cut them out of a Agarose gel - so I have seen the size- unless something happens after gel extraction. We...
Forum: Illumina/Solexa 11-06-2017, 10:28 PM
Replies: 13
Views: 1,851
Posted By ATGCT
Ah yes @ GenoMax - we spike with 5% PhiX. I thin...

Ah yes @ GenoMax - we spike with 5% PhiX. I thin we should increase to 10%
Forum: Illumina/Solexa 11-06-2017, 10:23 PM
Replies: 13
Views: 1,851
Posted By ATGCT
Hi, thanks for your answers !!! We are doing...

Hi, thanks for your answers !!!
We are doing Rad-seq libraries. Yes, I am quite sure, that my templates are bigger than 80bp- but we are checking this now using the bioanalyzer kit.
We already...
Forum: Illumina/Solexa 10-31-2017, 02:35 AM
Replies: 13
Views: 1,851
Posted By ATGCT
MiSeq v3 600

Hi everyone,

we have troubles with our MiSeq Runs. At about cycle 80 we have a massive decrease in Q30scores.In general the quality of the runs is not good. Q30 scores of 50 or lower. In addition,...
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