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Forum: Sample Prep / Library Generation 04-06-2018, 02:49 AM
Replies: 2
Views: 1,641
Posted By JamesHrastelj
?Over-dried AMPure beads?

Hi,

I have been using the same library prep kit. Although it's difficult to be sure exactly what has caused your problem, I know that one of the most sensitive parts of the protocol is the AMPure...
Forum: Sample Prep / Library Generation 03-09-2018, 02:26 AM
Replies: 4
Views: 3,555
Posted By JamesHrastelj
Repeat insertion with SMARTer Pico library prep

Hi,

I was very interested in the problems you've been having with the Clontech/Takara SMARTer Pico kit. I've just completed a pilot run of RNA-seq using 5 human total RNA samples (input 1-3ng,...
Forum: Introductions 03-09-2018, 01:18 AM
Replies: 0
Views: 1,120
Posted By JamesHrastelj
Live long and prosper

Hi all,

I'm a neurology clinical research registrar working in Cardiff, Wales, UK. I'm also a PhD student working in functional genetics of multiple sclerosis, a chronic neuroinflammatory...
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