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Forum: Bioinformatics 10-08-2020, 03:34 PM
Replies: 0
Views: 765
Posted By rtyagi
mitochondria in single nuclear RNAseq

for snRNAseq, do people use mitochondrial filtering? I'm not sure if this still makes sense even though we are working with data from nuclei rather than whole cells, but I do see some expression of...
Forum: Bioinformatics 04-28-2020, 12:18 PM
Replies: 0
Views: 535
Posted By rtyagi
issues with single nuclei sequencing

Hi All,

I am working on a set of single-nuclei sequencing samples. Does someone know why I might be getting a bimodal distribution in one of my samples (both in features/cell and reads/cell)? The...
Forum: Bioinformatics 07-06-2015, 06:10 AM
Replies: 4
Views: 1,321
Posted By rtyagi
No. not a single genome. just a very large...

No. not a single genome. just a very large reference set including many difference genomes. The reads come from a microbiome project.
Forum: Bioinformatics 07-05-2015, 06:19 PM
Replies: 4
Views: 1,321
Posted By rtyagi
Question bowtie with humongous reference database

Hi all,

I have been trying to find a way to build bowtie2 index for a really large reference database (>100GB) but I keep running into memory limits (obviously). Is there a way to do this (in...
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