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Forum: Illumina/Solexa 09-09-2016, 08:10 AM
Replies: 8
Views: 3,588
Posted By rEDI
Thank you again for your very helpful answers

Thank you again for your very helpful answers
Forum: Illumina/Solexa 09-09-2016, 08:05 AM
Replies: 8
Views: 3,588
Posted By rEDI
Thank you Fanli. In this case is it however that...

Thank you Fanli. In this case is it however that only 211 bases are covered by both?
Forum: Illumina/Solexa 09-09-2016, 07:58 AM
Replies: 8
Views: 3,588
Posted By rEDI
That is perfect, thank you so much for explaining...

That is perfect, thank you so much for explaining Fanli. That is most helpful

One more question - do the seqs unique to R1 and R2 not merge in this case, or are they merged regardless?

Thank...
Forum: Illumina/Solexa 09-09-2016, 07:41 AM
Replies: 8
Views: 3,588
Posted By rEDI
Hi Fanli Thank you for your answer The...

Hi Fanli

Thank you for your answer

The primer seqs are as follows:

forward
GTGCCAGCCGCCGCGGTAA

reverse
Forum: Illumina/Solexa 09-09-2016, 03:24 AM
Replies: 8
Views: 3,588
Posted By rEDI
Merging illumina V4 paired end reads

Hi

I am having difficulty understanding how my merged reads are producing a certain amplicon size.

Basically, I have 2 x 251bp reads. This 251bp includes primer sequence, of as far as I...
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