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Forum: Epigenetics 07-08-2012, 08:38 PM
Replies: 2
Views: 5,152
Posted By Liting
Why I can't open the Links? Can you give me...

Why I can't open the Links?
Can you give me the new links?Thanks !
Forum: Illumina/Solexa 06-27-2012, 07:36 AM
Replies: 3
Views: 2,341
Posted By Liting
Hi,mitchels I am tring the gel-size selection...

Hi,mitchels
I am tring the gel-size selection following Illumina adaptor ligation prior to commemcing the LM-PCR step in the NimbleGen in-solution Exon capture protocol。But we can't get enough PCR...
Forum: General 12-05-2011, 05:11 PM
Replies: 14
Views: 5,571
Posted By Liting
Hi Madseq, Thank you very much.We have just...

Hi Madseq,
Thank you very much.We have just noticed that the concentration of the sample makes a lot of a difference in the final fragment size.We have tried different conditions.The bioruptor is...
Forum: General 11-30-2011, 05:01 PM
Replies: 14
Views: 5,571
Posted By Liting
Thanks a lot.

Thanks a lot.
Forum: General 11-30-2011, 12:11 AM
Replies: 14
Views: 5,571
Posted By Liting
hello,Hamid.We also use the bioruptor to shear...

hello,Hamid.We also use the bioruptor to shear the genomic DNA.We want a tight product of 200-400bp.Can you give me some advice or protocal?The instrument in our lab is diogenode UCD 600.
Thank you...
Forum: Illumina/Solexa 11-29-2011, 10:40 PM
Replies: 8
Views: 7,109
Posted By Liting
hi Iterhune,we recently used Bioruptor to creat ...

hi Iterhune,we recently used Bioruptor to creat genomic DNA fragments about 300-400bp,but it doesn't concentrate. Can you give me some advice that I can get a concentrated fragments.The Bioruptor...
Forum: Sample Prep / Library Generation 11-09-2011, 02:07 AM
Replies: 6
Views: 2,930
Posted By Liting
Hello,sehrrot.I also have a problem about the PCR...

Hello,sehrrot.I also have a problem about the PCR richment during library preparation。I also have got some unexpected fragments which are about 100bp smaller than the expected fragments after PCR。...
Forum: Sample Prep / Library Generation 07-26-2011, 12:15 AM
Replies: 4
Views: 4,721
Posted By Liting
Hello,Monad.I have some problems and hope for...

Hello,Monad.I have some problems and hope for your help.Thank you.I used 20ug DNA for end repair for 6kb insert mate pair.The protocal is :the DNA with cycling adaptors,size selection after...
Forum: SOLiD 07-25-2011, 10:57 PM
Replies: 15
Views: 4,884
Posted By Liting
Hello

hello,Lovebv.I constructed a mate pair library at the first time.The SOP is similar to 454 library pre protocol.But the size of the library I constructed was between 100bp-200bp.I cann't figure out...
Forum: SOLiD 07-25-2011, 10:55 PM
Replies: 15
Views: 4,884
Posted By Liting
Hello everyone,I constructed a mate pair library...

Hello everyone,I constructed a mate pair library at the first time.The SOP is similar to 454 library pre protocol.But the size of the library I constructed was between 100bp-200bp.I wanted size of...
Forum: Illumina/Solexa 07-18-2011, 10:07 PM
Replies: 16
Views: 11,096
Posted By Liting
Library preparation

Thank you very much.Our adapters work well and don't have any problems.We have got enough libraries by PCR.But the size of DNA were smaller.It's abnormal.I can't find how it happens.We already...
Forum: Illumina/Solexa 07-10-2011, 10:48 PM
Replies: 16
Views: 11,096
Posted By Liting
We have had the same problem.We can not get...

We have had the same problem.We can not get enough libraries after PCR .We have confirmed that there is noting wrong with the ligase by ligating marker DNA.We think the problem is because of...
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