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Forum: RNA Sequencing 04-26-2018, 05:34 AM
Replies: 1
Views: 1,177
Posted By RevTK
Your data is most likely reverse stranded if the...

Your data is most likely reverse stranded if the kit uses dUTP method.
Confirm this by looking at your reads in IGV. For this, index your .bam files with e.g. samtools (command: samtools index...
Forum: Sample Prep / Library Generation 08-16-2017, 11:54 PM
Replies: 270
Views: 105,758
Posted By RevTK
We tried to make another library with the...

We tried to make another library with the mammalian pico kit, but the single-stranded DNA in my samples served as template for the RT used in that kit and we got mostly amplification of that.
We...
Forum: Sample Prep / Library Generation 04-03-2017, 12:20 AM
Replies: 270
Views: 105,758
Posted By RevTK
Dear all, I`m working with laser-capture...

Dear all,
I`m working with laser-capture microdissected cells (e.g. isolated a few hundred cell equivalents from frozen tissue sections) from mouse tissue. I`m using the single-cell lysis buffer...
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