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Forum: SOLiD 11-17-2010, 06:01 AM
Replies: 4
Views: 1,753
Posted By EADIE
Thanks for your information!

Thanks for your information!
Forum: RNA Sequencing 11-17-2010, 05:59 AM
Replies: 1
Views: 1,900
Posted By EADIE
Nobody has any ideas?

Nobody has any ideas?
Forum: SOLiD 11-16-2010, 12:43 AM
Replies: 4
Views: 1,753
Posted By EADIE
KevinLam, thanks for your advice!

KevinLam, thanks for your advice!
Forum: Bioinformatics 11-16-2010, 12:40 AM
Replies: 0
Views: 925
Posted By EADIE
how to exclude the known ncRNA

I have some data of Arabidopsis for small RNA analysis. Now i want to do a locall alignment with Rfam then exclude the sequences which match to Rfam from futher analysis. But the question is:
Do i...
Forum: SOLiD 11-14-2010, 05:56 AM
Replies: 4
Views: 1,753
Posted By EADIE
how to exclude the known ncRNA

I have some data of Arabidopsis for small RNA analysis. Now i want to do a locall alignment with Rfam then exclude the sequences which match to Rfam from futher analysis. But the question is:
Do i...
Forum: RNA Sequencing 11-14-2010, 05:33 AM
Replies: 1
Views: 1,900
Posted By EADIE
Rfam

I have some data of Arabidopsis for small RNA analysis. Now i want to do a locall alignment with Rfam then exclude the sequences which match to Rfam from futher analysis. But the question is:
Do i...
Forum: RNA Sequencing 11-14-2010, 05:30 AM
Replies: 7
Views: 11,029
Posted By EADIE
Rfam

I have some data of Arabidopsis for small RNA analysis. Now i want to do a locall alignment with Rfam then exclude the sequences which match to Rfam from futher analysis. But the question is:
Do i...
Forum: SOLiD 09-16-2010, 03:04 AM
Replies: 3
Views: 3,762
Posted By EADIE
coding exons/ repeats/ rRNA, tRNA, snRNA, snoRNA

hello,
i have some small RNA reads data of Arabidopsis thaliana. i have mapped them to Arabidopsis thaliana genome. now i want to exclude these reads(coding exons/ repeats/ rRNA, tRNA, snRNA,...
Forum: SOLiD 08-27-2010, 11:19 PM
Replies: 6
Views: 3,356
Posted By EADIE
Smile can RNA2MAP have the function of removing...

can RNA2MAP have the function of removing adapters and producing data of csfasta format?
bother you again...
Forum: SOLiD 08-25-2010, 01:23 AM
Replies: 6
Views: 3,356
Posted By EADIE
thanks for your reply! it helps me a lot... ...

thanks for your reply! it helps me a lot...
does bioscope or CLCBio is free? or should i write a perl script to remove adapters? is there any other free tools to do that?
Forum: SOLiD 08-22-2010, 01:05 AM
Replies: 6
Views: 3,356
Posted By EADIE
SOLID reads

hi,
i wonder to know what should i do before mapping reads to reference genome?
reads is like:
>853_1434_503_F3
T00010230120200223112313302010303131
>853_1434_640_F3...
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