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Forum: Sample Prep / Library Generation 08-22-2014, 05:25 AM
Replies: 0
Views: 1,410
Posted By ehdezsanabria
Exclamation How to deplete RNA without RiboZero or Terminator exonuclease?

Hello everyone!
Thank you for reading my question! Please excuse my little experience in the topic, I would like to have some advice on the RNA-seq library preparation. We extracted the RNA from...
Forum: Sample Prep / Library Generation 08-22-2014, 05:18 AM
Replies: 5
Views: 4,546
Posted By ehdezsanabria
Hi Jakob, We used the Turbo DNase from Ambion...

Hi Jakob,
We used the Turbo DNase from Ambion and followed by clean-up with RNAeasy mini kit. Then we used the Terminator exonuclease (because we do not have access to the RiboZero kit) and...
Forum: Sample Prep / Library Generation 08-22-2014, 05:05 AM
Replies: 18
Views: 12,989
Posted By ehdezsanabria
Hi chadn 737, We did the DNA removal using the...

Hi chadn 737,
We did the DNA removal using the Turbo DNase from Ambionbut used phenol:chloroform to inactivate the enzyme. Do you think this step is necessary or else, can you proceed directly to...
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