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Forum: Sample Prep / Library Generation 05-04-2018, 05:47 AM
Replies: 13
Views: 4,856
Posted By MU Core
chanwu, We trust both measurements but use...

chanwu,

We trust both measurements but use each for different library types. qPCR is used for PCR-free library preps with all other libraries which undergo amplification being measured by Qubit. ...
Forum: Sample Prep / Library Generation 07-01-2016, 06:12 AM
Replies: 13
Views: 4,856
Posted By MU Core
Consistency can be an issue. Our group performs...

Consistency can be an issue. Our group performs two independent HS Qubit readings per library. Doing so will help catch any outlier measurements due to a pipetting error, etc. The average is then...
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