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Forum: Sample Prep / Library Generation 06-30-2016, 02:44 PM
Replies: 13
Views: 4,857
Posted By nucacidhunter
Qubit and qPCR quantify different properties of...

Qubit and qPCR quantify different properties of library. Qubit quantifies dsDNA regardless of adapter presence in fragment termini while qPCR quantifies the fragments flanked by adapters that are...
Forum: Sample Prep / Library Generation 06-29-2016, 03:36 PM
Replies: 13
Views: 4,857
Posted By nucacidhunter
It is most likely due to concentration of...

It is most likely due to concentration of library. HS assay is recommended for library quantification because of its accuracy in low range.
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