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Search: Posts Made By: rcrystal
Forum: Bioinformatics 04-29-2015, 12:50 PM
Replies: 3
Views: 878
Posted By rcrystal
I also want to clarify, in case it's unclear. ...

I also want to clarify, in case it's unclear.
The experiment is set up like this:

Illumina RNASeq tissue of interest vs. Illumina RNASeq control tissue= RNASeq differentially expressed genes
...
Forum: Bioinformatics 04-29-2015, 12:44 PM
Replies: 3
Views: 878
Posted By rcrystal
Thanks for the reply, cmbetts! We're...

Thanks for the reply, cmbetts!

We're interested in documenting the differences in methodologies you're describing because we want to be aware of all of the caveats present using 3' DGE methods in...
Forum: Bioinformatics 04-29-2015, 11:50 AM
Replies: 3
Views: 878
Posted By rcrystal
Help with experiment analysis- comparison of RNASeq and 3' DGE NuGEN reads

Using a de-novo transcriptome assembled with Trinity, we are looking at differentially expressed genes between a control tissue and a tissue-of-interest in a spider.

Specifically, we are ...
Forum: Bioinformatics 06-28-2014, 07:47 AM
Replies: 12
Views: 5,656
Posted By rcrystal
Thanks maubp! I see it now, I'm sorry I missed it...

Thanks maubp! I see it now, I'm sorry I missed it before!
Forum: Bioinformatics 06-26-2014, 05:04 PM
Replies: 12
Views: 5,656
Posted By rcrystal
In case it helps anyone, I ran into this same...

In case it helps anyone, I ran into this same issue and found this blogger who states that the error messages are irrelevant:
...
Forum: RNA Sequencing 10-10-2013, 02:14 PM
Replies: 9
Views: 8,787
Posted By rcrystal
Hi- the paper that introduces RSEM (Li and...

Hi- the paper that introduces RSEM (Li and Dewey(2011). RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinformatics. 12: 323.) suggests that...
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