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Forum: Illumina/Solexa 04-21-2015, 01:34 PM
Replies: 1
Views: 1,632
Posted By Cyrus Taheri
Question Miseq custom primer design, primer linker

Miseq custom primer design, primer linker

Many papers have used a 2bp primer linker in 5 end of the amplicon primers. As, I understand this is to prevent taxon specific PCR bias due to matches to...
Forum: Illumina/Solexa 03-16-2015, 10:45 AM
Replies: 12
Views: 4,067
Posted By Cyrus Taheri
Thank you so much! I am eager to learn how your...

Thank you so much! I am eager to learn how your ITS sequencing trials go, good luck!

Thank you,
Forum: Illumina/Solexa 03-11-2015, 10:59 AM
Replies: 12
Views: 4,067
Posted By Cyrus Taheri
Hi kmcarr, this is promising. May I ask you which...

Hi kmcarr, this is promising. May I ask you which chemistry are you using, 500 cycles v2 or 600 cycles v3 and how many cycles you run? Did you bioanalyze your final library, if yes what was the size...
Forum: Illumina/Solexa 03-10-2015, 02:31 PM
Replies: 12
Views: 4,067
Posted By Cyrus Taheri
You are right clustering tend to be biased toward...

You are right clustering tend to be biased toward smaller amplicons. Knowing that miseq platform has been around for a few years now, I am also a bit surprised why only few papers are published on...
Forum: Illumina/Solexa 03-10-2015, 10:33 AM
Replies: 12
Views: 4,067
Posted By Cyrus Taheri
Hi tjpott, no I have not tried Miseq on fungal...

Hi tjpott, no I have not tried Miseq on fungal ITS yet. What are your primers?
Forum: Illumina/Solexa 10-01-2014, 08:03 AM
Replies: 12
Views: 4,067
Posted By Cyrus Taheri
Thank you!

Thank you!
Forum: Illumina/Solexa 09-29-2014, 01:34 PM
Replies: 12
Views: 4,067
Posted By Cyrus Taheri
Hi nucacidhunter, thank you for your advise. I am...

Hi nucacidhunter, thank you for your advise. I am more concerned if lower TM could affect the sequencing procedure in MiSeq. I agree that it is possible to adjust annealing temperature in first PCR,...
Forum: Illumina/Solexa 09-25-2014, 09:12 AM
Replies: 12
Views: 4,067
Posted By Cyrus Taheri
Question Illumina Miseq primer TM and library preparation for fungal metagenomics

I have previously worked with 454, but fairly new to Miseq platform. Using Nextera XT sample preparation kit, I am going to study soil and root fungal communities using fungal specific primers with...
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