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Forum: Metagenomics 05-24-2018, 07:27 AM
Replies: 2
Views: 5,335
Posted By MiniMicrobe
Hi Mastal Thanks for the reply. I thought...

Hi Mastal

Thanks for the reply. I thought that might be the case, I'm just trying to understand the data better.

I managed to find a bit of a tutorial about this too.
...
Forum: Metagenomics 05-22-2018, 07:11 AM
Replies: 2
Views: 5,335
Posted By MiniMicrobe
FastQC results for 16s reads

Hi

I have some FastQC results I have generated from Illumina 16s sequences. I have attached an example of the results for one of the demultiplexed fastq files, but they all look simillar.

I...
Forum: Bioinformatics 10-21-2017, 07:40 AM
Replies: 4
Views: 1,928
Posted By MiniMicrobe
Thank you gconcepcion, I had some time booked to...

Thank you gconcepcion, I had some time booked to day for the bioinfomatics computer and tried your suggestions. It worked perfectly!
I didn't know that BWA accepted pacbio reads for mapping. I...
Forum: Bioinformatics 10-17-2017, 01:26 PM
Replies: 4
Views: 1,928
Posted By MiniMicrobe
Hi Genomax Thanks for your reply. Using mauve...

Hi Genomax
Thanks for your reply. Using mauve is how I initially found the inversion. My supervisors want to know what the coverage was over the break points is to prove that there wasn't a miss...
Forum: Bioinformatics 10-17-2017, 11:28 AM
Replies: 4
Views: 1,928
Posted By MiniMicrobe
BBmap alignment error when using Pacbio reads

Hello

I am trying to perform mapping using pacbio sequences on a whole genome sequence. The back story is that i have produced a whole genome sequence with PacBio and Illumina miseq reads using...
Forum: Pacific Biosciences 12-09-2015, 08:01 AM
Replies: 1
Views: 1,785
Posted By MiniMicrobe
Should I try hybrid assembly with my PacBio data?

Hi all,

I recently had the genome of a bacterial strain I am working with sequenced using both PacBio and Illumina paired end.

I have managed to assemble the Illumina data into ~200 contigs...
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