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Forum: Introductions 06-23-2016, 02:05 PM
Replies: 10
Views: 3,352
Posted By buckiseq
We also have no problem with putting other...

We also have no problem with putting other libraries on both sequencers. The Fluidigm libraries were tough because they require a different sequencing primer from what is in the cartridges...
Forum: Introductions 03-11-2016, 06:10 AM
Replies: 10
Views: 3,352
Posted By buckiseq
luc, Illumina should be able to provide you...

luc,

Illumina should be able to provide you with the sequence- I am not sure about distributing it myself. We contacted Illumina about our issues with Fluidigm AA libraries on the NextSeq and they...
Forum: Introductions 03-09-2016, 07:19 AM
Replies: 10
Views: 3,352
Posted By buckiseq
imd, no worries! I forgot to mention that we...

imd, no worries! I forgot to mention that we started by using the v1 NextSeq kits as v2 was not for sale yet, but we switched to the v2 NextSeq kits and had much better success all around. We also...
Forum: Sample Prep / Library Generation 03-08-2016, 09:18 AM
Replies: 28
Views: 11,801
Posted By buckiseq
Just curious, what instruments are you all using...

Just curious, what instruments are you all using for the KAPA qPCR? We are using our QIAGEN Rotor-Gene Q and have enjoyed some pretty good success. We also have the option of using a 7500 Fast Dx and...
Forum: Introductions 02-29-2016, 05:26 AM
Replies: 10
Views: 3,352
Posted By buckiseq
Library type, Sequencing Primers, etc. ?

imd,

Sorry for the delay in my response. Read quality, particularly read two, was among the several problems when making the transition from the MiSeq to the NextSeq. Read two has been...
Forum: Introductions 01-06-2016, 06:56 AM
Replies: 10
Views: 3,352
Posted By buckiseq
Smile Hello SEQanswers! (MiSeq/NextSeq)

Hello!

I currently work at a laboratory helping to design, develop, validate, and run Next-Gen panels. We started with a MiSeq and have moved to a NextSeq, both using a Fluidigm based library...
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