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Forum: Illumina/Solexa 11-25-2017, 07:03 PM
Replies: 12
Views: 937
Posted By austinso
Doing this for a clinical lab is always a...

Doing this for a clinical lab is always a caveat...

Anyhow, I think all you would need to do is remove/comment out the lines from the Sony.xml file that is in the Exposures folder corresponding to...
Forum: Illumina/Solexa 10-18-2017, 03:31 PM
Replies: 12
Views: 937
Posted By austinso
Essentially, yes. Either a fixed number of random...

Essentially, yes. Either a fixed number of random bases (e.g. 5'-end of primer), or adding 0,+1, +2 bases of known composition.
Forum: Illumina/Solexa 10-18-2017, 12:38 PM
Replies: 12
Views: 937
Posted By austinso
Ultimately, the caveat is that the first few...

Ultimately, the caveat is that the first few bases are used as the QC step to identify successful cluster generation. So having dark cycles may not be in your best interest. But again depends on your...
Forum: Illumina/Solexa 10-18-2017, 11:09 AM
Replies: 12
Views: 937
Posted By austinso
Been a while since I've done this on the MiSeq,...

Been a while since I've done this on the MiSeq, and I know they've consistently updated these files to make it harder to modify...

All the protocols have traditionally been in XML files. So if you...
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