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Forum: Illumina/Solexa 11-30-2015, 01:22 PM
Replies: 13
Views: 5,312
Posted By kmcarr
It just occurred to me that we recently ran some...

It just occurred to me that we recently ran some custom amplicon libraries submitted by a researcher in which they used dual 12 bp indexes. It was a PE250 run so that is 502 cycles (251+251) plus the...
Forum: Illumina/Solexa 11-30-2015, 01:09 PM
Replies: 13
Views: 5,312
Posted By kmcarr
All Illumina sequencing kits (HiSeq SBS or MiSeq...

All Illumina sequencing kits (HiSeq SBS or MiSeq cartridges) include sufficient reagents for an additional 25 cycles beyond what their stated size is (e.g. 150 cycle kits have reagents sufficient for...
Forum: Illumina/Solexa 11-30-2015, 12:08 PM
Replies: 13
Views: 5,312
Posted By kmcarr
Dark cycles are only used prior to index read #2...

Dark cycles are only used prior to index read #2 in dual indexed runs. There would have been no dark cycles in the setup you described.

The "dark" or chemistry only cycles are used to extend the...
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