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Forum: Illumina/Solexa 05-20-2011, 07:23 AM
Replies: 8
Views: 3,340
Posted By antoniou
HI I think we have a similar problem, but just...

HI
I think we have a similar problem, but just t make sure, what are you calling "contamination". Are you talking about inward mapping reads?
Eric
Forum: Sample Prep / Library Generation 09-29-2010, 11:57 AM
Replies: 28
Views: 9,753
Posted By antoniou
I Agree. The Kappa kits have been great. our...

I Agree. The Kappa kits have been great. our cluster numbers are very consistent since we started using the kit (6 months ago)
Eric
Forum: RNA Sequencing 06-14-2010, 04:45 AM
Replies: 4
Views: 2,532
Posted By antoniou
Thanks Do you start from total RNA or do you...

Thanks
Do you start from total RNA or do you purify the miRNA using an acrylamide gel first?
Eric
Forum: RNA Sequencing 06-11-2010, 06:26 AM
Replies: 4
Views: 2,532
Posted By antoniou
making miRNA libraries

Hi
Does anyone have tried the Bioo Scientific AIRô Small RNA Sequencing Kit? It is quite a bit cheaper than the Illumina V1.5 kit and looks very similar.

Eric
Forum: Bioinformatics 06-07-2010, 08:01 AM
Replies: 365
Views: 159,895
Posted By antoniou
I just came across a reference to the following...

I just came across a reference to the following article in a different thread.
http://www.ncbi.nlm.nih.gov/pubmed/20395217?dopt=Citation
It also attributes the biases to random priming.


Eric
Forum: Bioinformatics 06-07-2010, 07:07 AM
Replies: 365
Views: 159,895
Posted By antoniou
I have seen the same phenomena but only with our...

I have seen the same phenomena but only with our mRNA-Seq libraries. Our genomic libraries do not show any biases. Has anyone else experienced this? Could it be an artifact of the Illumina library...
Forum: Illumina/Solexa 11-02-2009, 08:48 AM
Replies: 5
Views: 2,568
Posted By antoniou
The latest protocol on the Icon web site is dated...

The latest protocol on the Icon web site is dated from Sept 2009. I don't have the 2008 protocol so I cannot comment on the differences
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