SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 9 of 9
Search took 0.00 seconds.
Search: Posts Made By: dtm2451
Forum: Sample Prep / Library Generation 09-02-2014, 06:41 AM
Replies: 11
Views: 5,588
Posted By dtm2451
My lab also gets fairly hot in the Summer due to...

My lab also gets fairly hot in the Summer due to inefficient AC. Perhaps that was the issue as I'm no longer having SPRI trouble either. I don't like the heat explanation... I really don't get why...
Forum: Sample Prep / Library Generation 08-11-2014, 07:48 AM
Replies: 11
Views: 5,588
Posted By dtm2451
I'm not certain what might be going on here, but...

I'm not certain what might be going on here, but if MQ seems to work for the elution, could you just elute in water and then dilute in elution buffer?
Forum: Sample Prep / Library Generation 08-11-2014, 07:43 AM
Replies: 5
Views: 2,453
Posted By dtm2451
Hi Janaseq, It's very annoying when these...

Hi Janaseq,

It's very annoying when these things happen in protocols that you feel like an expert with isn't it? I think our issues might be slightly different, however, because your binding...
Forum: Sample Prep / Library Generation 08-11-2014, 07:37 AM
Replies: 5
Views: 2,453
Posted By dtm2451
Thanks for the suggestion. I called tech support...

Thanks for the suggestion. I called tech support last week. TS said they have never had a case like mine where the beads would not bind tubes at all in the first step (1st DNA binding step, before...
Forum: Sample Prep / Library Generation 08-07-2014, 08:45 AM
Replies: 5
Views: 2,453
Posted By dtm2451
Ampure XP beads that do not stick to wall of plate

Hello,

I am currently having an issue that I have never seen before with my ampure bead purifications. The beads are not sticking sufficiently to the sides of my wells, and an inconsistent but...
Forum: Sample Prep / Library Generation 07-30-2014, 12:30 PM
Replies: 282
Views: 139,095
Posted By dtm2451
Thank you jwfoley. I am actually thinking of...

Thank you jwfoley. I am actually thinking of trying both and testing biotin vs. iso vs. unblocked. But, I'm not sure how to get an oligo with both iso and LNA. Looking back, you actually mentioned...
Forum: Sample Prep / Library Generation 07-30-2014, 09:26 AM
Replies: 282
Views: 139,095
Posted By dtm2451
Hi Simone, Thanks very much for all of your...

Hi Simone,

Thanks very much for all of your comments and recommendations in this thread.

I am working to test/optimize the Smart-seq2 protocol for a Tcell application and am wondering how you...
Forum: Sample Prep / Library Generation 03-21-2014, 09:23 AM
Replies: 15
Views: 9,709
Posted By dtm2451
Extra peaks in a bioanalyzer trace for library made from ePCR amplicon

Hello,

I am working on a deep sequencing protocol for a PCR amplicon (~650bp) using the TruSeq DNA PCR-Free Sample Preparation Kit and I am seeing extra peaks in my final bioanalyzer traces that...
Forum: Sample Prep / Library Generation 09-04-2013, 06:52 AM
Replies: 2
Views: 3,603
Posted By dtm2451
Question Library prep with sheared ssDNA

Does anyone know the integrity/makeup of the 5' and 3' ends of Covaris-sheared ssDNA? I am working on a library prep method for single stranded cDNA and am hoping to ligate adapters post shearing.
...
Showing results 1 to 9 of 9

 


All times are GMT -8. The time now is 05:32 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO