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Forum: Bioinformatics 07-17-2012, 03:13 PM
Replies: 1
Views: 2,543
Posted By beefeng123
How to use Fisher's exact test in R

I have some RNA-seq data for statistic analysis. I do not have biological replicate for the data, so could anyone can give me some suggestion how to compare the significant expressed genes between...
Forum: Bioinformatics 06-28-2012, 08:48 AM
Replies: 15
Views: 5,256
Posted By beefeng123
Hi, Simon, Thank you so much for your...

Hi, Simon,
Thank you so much for your reply. It is very important to have replication, and I am planing to do that soon.
For the price, actually most part of the cost is the library...
Forum: Bioinformatics 06-26-2012, 05:33 PM
Replies: 15
Views: 5,256
Posted By beefeng123
Thank you so much, chadn737! What's you said...

Thank you so much, chadn737! What's you said makes me feel confidence to ask my PI for another replicate.

I still have one question: I checked some papers published earlier, they did not have...
Forum: Bioinformatics 06-26-2012, 03:06 PM
Replies: 15
Views: 5,256
Posted By beefeng123
Thank you very much for your reply, chadn737! ...

Thank you very much for your reply, chadn737!

1. It is good to have biological replicates to do the analysis. Just I have a little bit more samples, if have multiple replications, it will be very...
Forum: Bioinformatics 06-26-2012, 02:04 PM
Replies: 15
Views: 5,256
Posted By beefeng123
RNA-seq analysis of DEGs by different software?

Recently I got some RNA-seq data for differential expression analysis. Because there is no biological rep, I tried different software, edgeR, DESeq and DEGseq, and they came with different results.
...
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