Forum: Metagenomics
11-06-2019, 01:29 AM
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Replies: 0
Views: 5,708
GC content bias in ATCC mock reads
Hi! I have samples sequenced with NextSeq for the experiment protocol setup. We tested different kits (Nextera, Kapa, Qiagen) and apart from the donor samples, we sequenced ATCC bacterial mix with...
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Forum: Illumina/Solexa
10-29-2019, 03:44 AM
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Replies: 7
Views: 4,687
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Forum: Illumina/Solexa
10-29-2019, 01:19 AM
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Replies: 7
Views: 4,687
Thanks a lot - I just ran FastQC on my samples...
Thanks a lot - I just ran FastQC on my samples and it indeed indicated that Illumina adapters are present. However, it's not clear to me which Illumina adapters sequences should I use for trimming -...
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Forum: Illumina/Solexa
10-28-2019, 01:19 AM
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Replies: 7
Views: 4,687
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Forum: Illumina/Solexa
10-23-2019, 04:31 AM
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Replies: 7
Views: 4,687
QIAseq FX DNA Library Kit adapters for trimming
Can anyone point me to the sequences of QIAseq FX DNA Library Kit for adapters trimming in preprocessing? Illumina has exact sequences for trimming included in their manual, but I can't seem to find...
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