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Forum: Oxford Nanopore 11-29-2021, 05:12 AM
Replies: 2
Views: 1,915
Posted By jgibbons1
To answer my own question, after thinking about...

To answer my own question, after thinking about this more...

This kit includes a fragmentation step, which would result in cDNA fragmentation and limit sequencing cDNA of full in tact isoforms....
Forum: Oxford Nanopore 11-19-2021, 10:51 AM
Replies: 2
Views: 1,915
Posted By jgibbons1
Using the rapid sequencing kit for cDNA

We haven't done RNA-seq with the minion yet, but have an extra Rapid Sequencing kit lying around. I am wondering if there's anything "wrong" with creating our own cDNA and then sequencing it with the...
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