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Forum: Bioinformatics 03-03-2011, 06:31 AM
Replies: 7
Views: 4,915
Posted By Vickenstein
Thanks for the reply. From the look of it I might...

Thanks for the reply. From the look of it I might have to reassemble the raw reads from 454 and the new reads from Illumina using both Oasis/velvet and Newbler. I will compare the results between...
Forum: Bioinformatics 03-01-2011, 06:44 AM
Replies: 7
Views: 4,915
Posted By Vickenstein
Assembling De Novo 454 Transcriptome Contigs and Singletons with Illumina Short Reads

Background: I have assembled 4 million None Normalized 454 Titanium de novo transcriptome Reads using Newbler 2.5, and have gotten amazing results, 88000 contigs with N50 of 951 bp, 35000 isotigs...
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