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Forum: Illumina/Solexa 04-27-2019, 03:15 PM
Replies: 1
Views: 707
Posted By emham
\_(ツ)_/

or is it just that READ1 is always OT/OB so that dictates the orientation for the entire read pair even though READ2 is the reverse complement of that?!
Forum: Illumina/Solexa 04-27-2019, 02:53 PM
Replies: 1
Views: 707
Posted By emham
Help understanding directional libraries in bisulfite sequencing

Hi all, I know there are some other threads about this and I have read them, but for some reason I'm still struggling to understand the molecular biology behind directional vs. non-directional...
Forum: Illumina/Solexa 04-26-2019, 12:24 AM
Replies: 3
Views: 1,429
Posted By emham
p5 does bind and form clusters but then the p5...

p5 does bind and form clusters but then the p5 molecules are cleaved and p7-attached clusters are sequenced first in read1. then those molecules are copied by bridge PCR and the p5-attached molecules...
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