SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 5 of 5
Search took 0.00 seconds.
Search: Posts Made By: Manal
Forum: RNA Sequencing 05-21-2013, 04:42 AM
Replies: 18
Views: 7,047
Posted By Manal
Hi susma sorry for late reply check the file...

Hi susma
sorry for late reply
check the file attached it might be useful, in our lab we are following Illumina protocols and have been provided with this when we had fragmentation problem.
Forum: RNA Sequencing 05-06-2013, 12:29 AM
Replies: 18
Views: 7,047
Posted By Manal
Actually i was having similar results, the 30sec...

Actually i was having similar results, the 30sec will make a difference and also in in my case i had problems with some of the adapters , i dodnt know what are the problems but they simply didnt...
Forum: RNA Sequencing 05-05-2013, 11:02 PM
Replies: 18
Views: 7,047
Posted By Manal
Ok then try to incubate it for 7:30min at 94C,...

Ok then try to incubate it for 7:30min at 94C, and see what will happen. and also for better practice as RNA is very sensitive work through the protocol and navigate from step to another while your...
Forum: RNA Sequencing 05-03-2013, 11:55 PM
Replies: 18
Views: 7,047
Posted By Manal
Hi Susma In my case I had to change the...

Hi Susma
In my case I had to change the incubation time ( in Illumina protocol, the fragmentation step should be done at 94C for 8min) but i have optimize it to 7:30min, and it made a big...
Forum: RNA Sequencing 05-01-2013, 12:09 AM
Replies: 18
Views: 7,047
Posted By Manal
Hi susma I just had a look at your libraries,...

Hi susma
I just had a look at your libraries, it seems that you had problem in the fragmentation of your RNA as you have small peaks within the range, I dont know how your protocol is but I am using...
Showing results 1 to 5 of 5

 


All times are GMT -8. The time now is 10:52 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO