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Search: Posts Made By: soungalo
Forum: Bioinformatics 04-22-2018, 02:46 AM
Replies: 13
Views: 1,987
Posted By soungalo
Wrong way, sir

You won't be able to get a de novo assembly this way.
Regarding the Illumina strategy - to get a decent assembly you'll have to:
a. Increase coverage (50-60X should be OK)
b. Use longer reads...
Forum: Bioinformatics 04-22-2018, 12:38 AM
Replies: 0
Views: 905
Posted By soungalo
Talking Data download from ENA is rather slow

I am trying to download some fastq data from ENA.
Using the ftp links with the wget command in Linux. Something like:
wget ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR410/ERR410229/ERR410229_2.fastq.gz...
Forum: 454 Pyrosequencing 04-22-2018, 12:18 AM
Replies: 5
Views: 7,691
Posted By soungalo
Cool 454 GS FLX Titanium 'paired end' protocol

Hi,
I know this is an obsolete technology, but I came across some data produced on the 454 instrument (archived in SRA/ENA) which I'd like to use for assembly.
According to the meta data, insert...
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