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Forum: Ion Torrent 04-19-2016, 03:51 PM
Replies: 8
Views: 7,128
Posted By owik303
we were loading less than 26pM routinely as a way...

we were loading less than 26pM routinely as a way to combat the affect of the aggerates, to as little as 10pM, this helped with polyclonal but had no affect on the short read fragments. The heatcool...
Forum: Introductions 04-19-2016, 03:07 PM
Replies: 4
Views: 4,739
Posted By owik303
This is super easy, we have done it multiple...

This is super easy, we have done it multiple times with no issue besides needing to buy a longer read on Illumina. Just take your ion torrent library as is and put it through the truseq kit, having...
Forum: Ion Torrent 04-19-2016, 02:59 PM
Replies: 8
Views: 7,128
Posted By owik303
mhgseq - we have resolved this issue greatly, I...

mhgseq - we have resolved this issue greatly, I have demonstrated on the same library short reads cannibalizing up to 70%+ of the total reads form a 314, 316 and 318 chips even with deceased loading...
Forum: Ion Torrent 08-20-2012, 08:18 AM
Replies: 8
Views: 7,128
Posted By owik303
Thanks for responding and I wold love to hear...

Thanks for responding and I wold love to hear what you have to say, I have updated my profile to receive emails from everyone now, please email away! If for whatever reason you still encounter a...
Forum: Ion Torrent 08-16-2012, 04:19 PM
Replies: 8
Views: 7,128
Posted By owik303
Question Amplicon Sequencing with Ion Torrent

Hi, I've been reading threads in this forum for months now and have found a lot of useful information, and seen many questions answered and figured id give it a try.

I am currently working on...
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