Forum: 454 Pyrosequencing
12-16-2010, 09:04 AM
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Replies: 15
Views: 4,305
It is the oil
We've had 50% enrichment with the new SV oils even after halving the cpb. Switched back to a stock of old oils last week and the problem disappeared. There is definitely something different about the...
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Forum: 454 Pyrosequencing
12-01-2010, 06:30 AM
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Replies: 15
Views: 4,305
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Forum: 454 Pyrosequencing
11-19-2010, 10:30 AM
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Replies: 11
Views: 3,913
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Forum: 454 Pyrosequencing
11-16-2010, 10:23 AM
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Replies: 15
Views: 4,305
emPCR issues
Enrichment following SV emPCR has quite dramatically increased with the last few preps. We're well used to doing a range of library preps and subsequent emPCR for both amplicon and shotgun using the...
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Forum: The Pipeline
11-02-2010, 02:53 AM
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Replies: 5
Views: 4,392
Exciting indeed eco
I think the next 2 years is going to be great viewing for the impartial observer - and this site will probably give a pretty good front row seat!
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Forum: The Pipeline
11-01-2010, 10:11 AM
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Replies: 5
Views: 4,392
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Forum: SOLiD
07-15-2009, 01:21 AM
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Replies: 7
Views: 4,607
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Forum: SOLiD
07-14-2009, 03:06 AM
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Replies: 7
Views: 4,607
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Forum: 454 Pyrosequencing
07-10-2009, 07:04 AM
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Replies: 1
Views: 2,126
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Forum: Literature Watch
07-02-2009, 09:36 AM
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Replies: 2
Views: 1,898
Genomics: catch me if you can
A nice summary of target specific sequencing methods
http://www.nature.com/nmeth/journal/v6/n7/pdf/nmeth0709-539.pdf
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Forum: Bioinformatics
06-04-2009, 04:17 AM
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Replies: 0
Views: 1,821
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Forum: 454 Pyrosequencing
04-17-2009, 01:23 AM
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Replies: 34
Views: 15,669
Hi Gerald
Are you doing a size exclusion...
Hi Gerald
Are you doing a size exclusion with the cDNA and a titration (concentrate the titration at the lower end)? Also, because fragment size is usually shorter for cDNA it may be worth...
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Forum: 454 Pyrosequencing
04-16-2009, 06:24 AM
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Replies: 34
Views: 15,669
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Forum: Bioinformatics
02-04-2009, 03:35 AM
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Replies: 8
Views: 4,267
What NextGen LIMS do you use?
I know "cquincer" started a very similar post to this back at the start of December last year. There wasn't a great deal of feedback at the time and I was wondering if anyone could offer some up to...
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Forum: The Pipeline
01-12-2009, 04:50 AM
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Replies: 4
Views: 7,206
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Forum: Site Announcements
11-20-2008, 04:10 AM
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Replies: 9
Views: 5,369
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Forum: 454 Pyrosequencing
11-08-2008, 06:10 AM
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Replies: 3
Views: 4,661
I saw this poster being presented at a recent...
I saw this poster being presented at a recent GS-FLX user’s group meeting in London. I got the impression that 454 Branford had primarily implemented this technology in order to scale up their sample...
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Forum: 454 Pyrosequencing
10-24-2008, 08:06 AM
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Replies: 0
Views: 2,700
Mixed fragment size normalized cDNA sequencing
I was just wondering what general approach and experience groups have had sequencing mixed fragment size cDNA (transcriptome sequencing LMW 70 – 500 bp AND > 1.5 kb in one sample). Not only that, but...
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Forum: 454 Pyrosequencing
10-08-2008, 08:02 AM
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Replies: 2
Views: 3,089
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