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Forum: Bioinformatics 09-26-2011, 10:59 PM
Replies: 1
Views: 4,078
Posted By f0415007
my command line: samtools mpileup -uf [ref]...

my command line:

samtools mpileup -uf [ref] [aln.bam] | /bcftools/bcftools view -cg - | /bcftools/vcfutils.pl vcf2fq > [result.fq]

the result shows :
@gi|110645304|ref|NC_002516.2|...
Forum: Bioinformatics 09-26-2011, 06:51 PM
Replies: 1
Views: 4,078
Posted By f0415007
Forum: General 09-21-2011, 06:41 PM
Replies: 1
Views: 2,247
Posted By f0415007
Question HELP, primer design problem in gap closure

hey guys, i'm newbie here.

i got a question in primer design.

now i've got 100 contigs and starting gap closure using PCR method. i've no idea how to design primer between contigs. using...
Forum: Illumina/Solexa 09-20-2011, 11:46 PM
Replies: 0
Views: 2,123
Posted By f0415007
Lightbulb HELP, primer design problem in gap closure

hey guys, i'm newbie here.

i got a question in primer design.

now i've got 100 contigs and starting gap closure using PCR method. i've no idea how to design primer between contigs. using...
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