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Forum: 454 Pyrosequencing 03-18-2013, 09:27 AM
Replies: 5
Views: 2,191
Posted By UofG
Do you omit the Sizing Sizing solution completely...

Do you omit the Sizing Sizing solution completely when performing the AMpure clean up? I have had mixed results using the Sizing Solution, and often wondered if I'd be better off without it.
Forum: 454 Pyrosequencing 02-01-2013, 12:34 PM
Replies: 21
Views: 9,429
Posted By UofG
I just put a sequencing run on with beads...

I just put a sequencing run on with beads combined from two runs that both resulted in low enrichment. I will know tomorrow.
Forum: 454 Pyrosequencing 02-01-2013, 10:22 AM
Replies: 21
Views: 9,429
Posted By UofG
I am relieved to see many people having the same...

I am relieved to see many people having the same problems as I have been experiencing the past two months. Well I am not happy that your work is being compromised, but I have been banging my head...
Forum: 454 Pyrosequencing 01-15-2013, 06:52 AM
Replies: 2
Views: 1,221
Posted By UofG
Has anybody used an expired PTP kit?

They have expiry dates, but it is only the plate and the rubber loading gasket and cartridge seal. I presume it would be fine, but I would appreciate any input from someone who has used an expired...
Forum: 454 Pyrosequencing 12-03-2012, 07:08 AM
Replies: 0
Views: 863
Posted By UofG
Stalled GS Junior Run

Hello everybody. I am new here, so I apologize if this is posted in the incorrect forum. Please feel free to direct me elsewhere if required.

I have been using the GS Junior platform for 6 months...
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