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Forum: Sample Prep / Library Generation 06-24-2019, 12:38 PM
Replies: 4
Views: 1,152
Posted By SNPsaurus
If they loaded PhiX at 5% but the library was...

If they loaded PhiX at 5% but the library was mis-estimated and actually much higher, then the actual PhiX could be lower, leading to poor quality scores.

Are you sequencing just one of the cut...
Forum: Sample Prep / Library Generation 06-19-2019, 12:00 PM
Replies: 4
Views: 1,152
Posted By SNPsaurus
Did the facility add extra PhiX to help the...

Did the facility add extra PhiX to help the basecalling in the low-complexity region of the cut site? What is the cut site?

It does seem like two issues. If you are getting lots of off-target...
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