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Forum: Ion Torrent 02-20-2019, 06:28 AM
Replies: 1
Views: 2,565
Posted By geejaytee
They do not need correcting - as the deamination...

They do not need correcting - as the deamination (C>T and G>A) damage is random, the predicted allele frequencies for these (false) calls will be small (<0.25, say), and will be of low quality. A...
Forum: Ion Torrent 02-05-2019, 05:55 AM
Replies: 1
Views: 2,565
Posted By geejaytee
Correcting for deamination in variant calling

Hiya,

I'm calling variants using the TorrentSuite on DNA which has been sequenced from formalin-fixed paraffin-embedded tissue. This has a major issue in that without addition of...
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