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Search: Posts Made By: demienx
Forum: Bioinformatics 12-17-2014, 10:27 PM
Replies: 5
Views: 1,822
Posted By demienx
Hi HESmith, I should apologize for making such...

Hi HESmith,
I should apologize for making such a simple mistake, and thank you for pointing it out for me. I originally thought that mem was suitable for a wider range of read length than backtrack.
Forum: Bioinformatics 12-17-2014, 10:11 PM
Replies: 5
Views: 1,822
Posted By demienx
Thank you Ihavenoidea. It seems very possible...

Thank you Ihavenoidea. It seems very possible that the low mapping rate is due to my short read length. But could you tell me how long your miRNA read is?
Forum: Bioinformatics 12-16-2014, 12:05 AM
Replies: 5
Views: 1,822
Posted By demienx
Question nothing aligned using bwa mem

Hi there.
I am using bwa to align human RNA-seq reads to rRNA database to exclude rRNA pollution. I tried both bwa backtrack and mem, and very strangely only backtrack could map some of my reads to...
Forum: Bioinformatics 10-27-2014, 11:56 PM
Replies: 1
Views: 2,126
Posted By demienx
Concerning your first question, my opinion is the...

Concerning your first question, my opinion is the positive length stands for deletion and the negative for insertion.
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