Forum: Sanger/Dye Terminator
10-08-2015, 08:57 AM
|
|
Replies: 3
Views: 6,347
|
|
Forum: Ion Torrent
10-22-2014, 08:12 PM
|
|
Replies: 22
Views: 29,422
|
|
Forum: Sample Prep / Library Generation
10-18-2013, 07:05 AM
|
|
Replies: 49
Views: 73,831
|
|
Forum: RNA Sequencing
06-24-2012, 11:20 AM
|
|
Replies: 3
Views: 3,840
RNA fragmentation
Hi:
What is your result with either Mg2+ or Zn2+ based buffer? I prefer Mg2+ based buffer but can't find any protocol. thanks,
|
|
Forum: Sample Prep / Library Generation
04-13-2012, 10:51 AM
|
|
Replies: 23
Views: 18,606
Sizeselector from Aline Bio
You may talk to Tufts University core facility. They have some good experience. It is very effective in removing adaptor contamination.
|
|
Forum: Illumina/Solexa
09-02-2010, 01:06 PM
|
|
Replies: 32
Views: 24,040
It is true that all the robots do is pipetting....
It is true that all the robots do is pipetting. However, if someone has spent the time writing up scripts and user interface and tuning up the process, it will really save a lot time and effort to...
|
|
Forum: Illumina/Solexa
09-01-2010, 06:36 PM
|
|
Replies: 32
Views: 24,040
I wonder who is working on automated library...
I wonder who is working on automated library construction that is an open system so that you don't have to be tied up to a particular vendor. Some institutes have their own high throughput library...
|
|
Forum: Sample Prep / Library Generation
05-04-2010, 08:55 PM
|
|
Replies: 3
Views: 2,474
|
|
Forum: Sample Prep / Library Generation
05-03-2010, 11:34 AM
|
|
Replies: 5
Views: 9,943
|
|
Forum: Sample Prep / Library Generation
05-03-2010, 11:32 AM
|
|
Replies: 5
Views: 9,943
|
|
Forum: Sample Prep / Library Generation
05-03-2010, 10:28 AM
|
|
Replies: 3
Views: 3,006
|
|
Forum: 454 Pyrosequencing
05-02-2010, 09:57 PM
|
|
Replies: 2
Views: 5,636
|
|
Forum: Illumina/Solexa
03-31-2010, 08:13 PM
|
|
Replies: 32
Views: 24,040
Cost is an issue. Once you have bought the...
Cost is an issue. Once you have bought the system, you are lock into the system. You have to keep buying whatever they offer. Another issue is that we all know the the genomics field is evolving...
|
|
Forum: Sample Prep / Library Generation
03-26-2010, 09:14 PM
|
|
Replies: 5
Views: 9,943
|
|
Forum: Illumina/Solexa
03-24-2010, 07:58 AM
|
|
Replies: 32
Views: 24,040
|
|
Forum: Illumina/Solexa
03-17-2010, 08:58 PM
|
|
Replies: 32
Views: 24,040
|
|
Forum: Illumina/Solexa
03-17-2010, 08:06 PM
|
|
Replies: 32
Views: 24,040
This machine has a few characteritics based on...
This machine has a few characteritics based on anecdotes I heard so far:
1, you get 10% yield, which means 3 to 4 more cycles of PCR to compensate for the loss. Lib bias may be generated from PCR....
|
|
Forum: Sample Prep / Library Generation
02-16-2010, 04:48 PM
|
|
Replies: 23
Views: 18,606
Check this out: ...
Check this out:
http://www.genomeweb.com/sage-science-automates-dna-fractionation-next-gen-sequencing.
Double selection means you do the same selection twice. You will lose more DNA with each...
|
|
Forum: Sample Prep / Library Generation
02-15-2010, 01:36 PM
|
|
Replies: 10
Views: 5,513
|
|
Forum: Illumina/Solexa
02-15-2010, 11:02 AM
|
|
Replies: 9
Views: 5,145
|
|
Forum: Sample Prep / Library Generation
02-14-2010, 08:33 AM
|
|
Replies: 27
Views: 12,280
Some of the DNA is damaged during shearing. My...
Some of the DNA is damaged during shearing. My experience has been under certain conditions, only half of the ends can be repaired on one molecule. I don't know the reason yet, but I think the DNA...
|
|
Forum: Sample Prep / Library Generation
02-14-2010, 08:13 AM
|
|
Replies: 17
Views: 9,844
|
|
Forum: Sample Prep / Library Generation
02-14-2010, 08:10 AM
|
|
Replies: 17
Views: 9,844
|
|
Forum: Sample Prep / Library Generation
02-14-2010, 07:58 AM
|
|
Replies: 23
Views: 18,606
The reason is that you have a lot DNA at 130....
The reason is that you have a lot DNA at 130. Ampure size selection is not as tight. When you have a lot DNA in the vicinity of your target size, it will just carry over. A double selection or...
|
|
Forum: Sample Prep / Library Generation
02-14-2010, 07:29 AM
|
|
Replies: 49
Views: 73,831
Ampure
Ampure is just more expensive. Some of the time saved is just from reducing drying time. I think PCRClean is better in terms of band tightness.
|
