Forum: General
02-20-2019, 07:02 AM
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Replies: 5
Views: 1,774
It sounds something like this:...
It sounds something like this: https://www.thermofisher.com/order/catalog/product/403081
From your description, I don't think that's the one you're looking for, but the terms there might get you...
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Forum: Sample Prep / Library Generation
02-20-2019, 06:45 AM
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Replies: 0
Views: 1,441
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Forum: Sample Prep / Library Generation
05-17-2018, 03:41 PM
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Replies: 1
Views: 1,280
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Forum: Sample Prep / Library Generation
04-03-2018, 07:09 AM
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Replies: 1
Views: 862
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Forum: Sample Prep / Library Generation
07-02-2017, 07:07 PM
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Replies: 3
Views: 2,899
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Forum: Sample Prep / Library Generation
07-02-2017, 06:59 PM
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Replies: 3
Views: 1,978
Optimizing magnetic bead mix for gDNA extraction
I've been working with the protocol in this thread for making magnetic bead mixes: http://seqanswers.com/forums/showthread.php?t=49507
I have it working well as a substitute for Ampure/SPRIselect,...
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Forum: Sample Prep / Library Generation
06-08-2017, 05:49 PM
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Replies: 3
Views: 2,899
qubit and picogreen discrepancies
We're a small lab that has done most of our quantitation using nanodrop and qubit, depending on the application. However, as we try to prepare multiplexed illumina libraries for dsDNA, we're trying...
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Forum: Sample Prep / Library Generation
06-05-2017, 07:30 AM
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Replies: 12
Views: 8,549
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Forum: Sample Prep / Library Generation
05-12-2017, 05:42 PM
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Replies: 2
Views: 1,384
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Forum: Sample Prep / Library Generation
05-11-2017, 04:54 PM
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Replies: 2
Views: 1,384
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Forum: General
03-10-2017, 11:02 AM
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Replies: 1
Views: 2,128
PstI methylation sensitivity
So my lab is in the process of understanding and implementing a protocol for several species. One point of confusion we are trying to figure out is whether or not the PstI restriction enzyme is...
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Forum: Pacific Biosciences
09-16-2016, 01:24 PM
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Replies: 5
Views: 2,835
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Forum: Pacific Biosciences
09-16-2016, 07:21 AM
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Replies: 5
Views: 2,835
I don't see anything a report called a loading...
I don't see anything a report called a loading report, or that gives p0, p1, p2 statistics. I've attached screenshots of the reports I do have. The left side is the problematic species. The right...
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Forum: Pacific Biosciences
09-14-2016, 10:15 AM
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Replies: 5
Views: 2,835
Low read counts from old PacBio data
I've been helping other people with processing PacBio data using the SMRTanalysis software. Basically, for our purposes, I've just been using the reads of insert protocol. This has worked great for...
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Forum: Pacific Biosciences
03-31-2016, 11:16 AM
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Replies: 1
Views: 2,039
Developing microsatellites from pacbio reads
Disclaimer: everything I know about Pacbio has been learned over the process of the last few days. I'm basically the closest thing to a bioinformatician in our lab, so I'm helping some people figure...
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