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Forum: Illumina/Solexa 07-25-2016, 11:11 PM
Replies: 9
Views: 3,356
Posted By cerbatana
Hi! I just wanted to show my PFGE results. As you...

Hi! I just wanted to show my PFGE results. As you and Illumina staff already pointed out, DNA 12000 chip cannot resolve these fragments, so I ran a PFGE and I can clearly see the fragmented DNA. The...
Forum: Illumina/Solexa 07-25-2016, 03:05 AM
Replies: 9
Views: 3,356
Posted By cerbatana
Thank you for the feedback. I was also thinking...

Thank you for the feedback. I was also thinking about the enzyme not being efficient enough, but in two different batches I saw it as a little bit unlikely. The funny thing is that I showed these...
Forum: Illumina/Solexa 07-24-2016, 10:34 PM
Replies: 9
Views: 3,356
Posted By cerbatana
Thanks a lot for your help. I was also told to...

Thanks a lot for your help. I was also told to try using TapeStation since Bioanalyzer could not render reliable results some times with fragments of these sizes (gel migration problems I assume) so...
Forum: Illumina/Solexa 07-21-2016, 05:13 PM
Replies: 9
Views: 3,356
Posted By cerbatana
Hi! I cleaned the tagmented dna with the zymo...

Hi! I cleaned the tagmented dna with the zymo columns and what I applied to the dna chip was diluted according to the protocol in 7 uL of water. Is it necessary an additional clean up step?...
Forum: Illumina/Solexa 07-21-2016, 08:44 AM
Replies: 9
Views: 3,356
Posted By cerbatana
Red face Nextera Mate Pair library: expected size after tagmentation

Hi! I'm having problems with the tagment genomic DNA step of the Nextera mate-pair library preparation kit and I really don't know if the enzyme is working at all. I' attaching the bioanalyzer DNA...
Forum: Sample Prep / Library Generation 07-21-2016, 08:18 AM
Replies: 14
Views: 12,282
Posted By cerbatana
Unhappy EDIT: I just realized that the original post...

EDIT:

I just realized that the original post was about Nextera kit while the one I' using is Nextera mate-pair kit. I'll create a new thread so this post can be removed. Thank you.

Hi! I'm also...
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