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Forum: Illumina/Solexa 03-20-2017, 04:55 AM
Replies: 6
Views: 1,157
Posted By seb42
Thank you for your answers. We do have a phiX...

Thank you for your answers. We do have a phiX spike in our runs and we are using a modified version of the Earth Microbiome Project. Our primers are a bit different than the ones mentioned in that...
Forum: Illumina/Solexa 03-19-2017, 08:35 AM
Replies: 6
Views: 1,157
Posted By seb42
Degenerate sequencing primers

Hello,

We are using custom sequencing primers containing multiple degenerate bases for MiSeq sequencing of the V4 SSU region. We have included these degenerate bases to hit more taxa during the...
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