Forum: Illumina/Solexa
10-13-2016, 06:39 AM
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Replies: 19
Views: 8,970
Hi everyone,
Thank you huguesparri for...
Hi everyone,
Thank you huguesparri for your input!
We had some meetings and my last informations are:
- Epmotion 5075 : The software looks easy to work, I thought it was more difficult....
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Forum: Illumina/Solexa
10-05-2016, 05:44 AM
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Replies: 19
Views: 8,970
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Forum: Illumina/Solexa
10-04-2016, 05:41 AM
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Replies: 19
Views: 8,970
Hi,
Our list just increase with 1 more...
Hi,
Our list just increase with 1 more brand, but we still have some questions/concerns that we're trying to get in contact with the tech support.
- Epmotion 5075 : In the beginning, they said...
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Forum: Illumina/Solexa
09-30-2016, 06:27 AM
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Replies: 19
Views: 8,970
Hello everyone,
Does anyone have more...
Hello everyone,
Does anyone have more information to update this post?
We're in the middle of the same process to choose a Liquid Handling Robot, and our list is:
- Hamilton NGS
-...
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Forum: Illumina/Solexa
05-06-2016, 10:16 AM
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Replies: 5
Views: 2,018
The replacement for this kit arrived today ...
The replacement for this kit arrived today
We'll receive another kit, from other Run, no more replacements because of the high cluster density :D
This kit its very stable even in overclustering...
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Forum: Illumina/Solexa
05-06-2016, 09:47 AM
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Replies: 5
Views: 2,018
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Forum: Illumina/Solexa
05-06-2016, 09:32 AM
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Replies: 5
Views: 2,018
Miseq V2 kit (2x250 cycles) drop quality
Hi,
We detected some drop quality (R2) on the Miseq V2 kit (2x250 cycles), anyone else?
p. ex. R1 finished arround 87% Q30 and R2 with 67%.
Is the V2 kit the new (old) V3 (2x300 cycles)?
...
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Forum: Illumina/Solexa
04-08-2016, 01:55 AM
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Replies: 68
Views: 27,175
I got one of that cartridges :)
Our FAS said...
I got one of that cartridges :)
Our FAS said that Illumina wants to release "the new" V3 kit after Summer.
The last V3 kit we used as a V2 2x250 cycles, the difference is that the V3 got more...
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Forum: Illumina/Solexa
12-22-2015, 03:53 AM
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Replies: 4
Views: 2,661
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Forum: Illumina/Solexa
12-18-2015, 04:03 PM
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Replies: 4
Views: 2,661
MiSeq Failed Run
Hello,
I got this:
"Your run xxxxxxxx on instrument xxxxxxx recently encountered the following error during sequencing:
<Internal Illumina Command Exception>"
Any ideas?
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Forum: Illumina/Solexa
11-12-2015, 02:27 AM
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Replies: 68
Views: 27,175
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Forum: Core Facilities
10-14-2015, 05:53 AM
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Replies: 4
Views: 8,014
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Forum: Illumina/Solexa
07-09-2015, 01:27 AM
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Replies: 68
Views: 27,175
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Forum: Illumina/Solexa
06-29-2015, 04:06 PM
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Replies: 68
Views: 27,175
Hello everybody,
The quality drop its a real...
Hello everybody,
The quality drop its a real issue, we already report to Illumina 4-5 Runs, with more or less the same metrics (last one R2=52%Q30). We got cluster density from 900 to 1400 k/mm2...
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Forum: Sample Prep / Library Generation
05-28-2015, 09:01 AM
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Replies: 20
Views: 9,463
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Forum: Illumina/Solexa
05-28-2015, 08:49 AM
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Replies: 16
Views: 6,042
Hi Vinz,
Thank you for your post.
It was...
Hi Vinz,
Thank you for your post.
It was me that opened this discussion about the contamination, just because we detected some biofilm in the manual sipper. And after the new non-official bleach...
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Forum: Illumina/Solexa
05-05-2015, 04:15 PM
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Replies: 16
Views: 6,042
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Forum: Illumina/Solexa
04-21-2015, 05:38 AM
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Replies: 16
Views: 6,042
Hi nangel,
Thank you for your reply
...
Hi nangel,
Thank you for your reply
Now there´s some metrics that make sense, the prephasing increase, Q scores problems etc. I'm gona check the last runs to confirm this.
Now the...
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Forum: Illumina/Solexa
04-15-2015, 07:56 AM
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Replies: 16
Views: 6,042
MiSeq fungal contamination
Hello,
We detected some kind of biofilm on the Miseq manual sipper and have reported to Illumina support. The reply from the supposed Illumina support was a little bit unsupported. They said that...
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Forum: Illumina/Solexa
09-14-2014, 10:31 AM
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Replies: 55
Views: 32,806
Is this what you are looking for?
...
Is this what you are looking for?
http://supportres.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf
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Forum: Illumina/Solexa
07-22-2014, 02:11 AM
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Replies: 2
Views: 2,001
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Forum: Illumina/Solexa
07-15-2014, 08:15 AM
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Replies: 3
Views: 2,935
Hi dfhdfh,
Thank you for your feedback.
We...
Hi dfhdfh,
Thank you for your feedback.
We alreday runned the Miseq test and there's no problem in the fluidic system, but our local support will be here tomorrow to change some part from the...
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Forum: Illumina/Solexa
07-13-2014, 09:37 AM
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Replies: 3
Views: 2,935
Bubbles in Miseq Flowcell
Hi everyone,
I would like to know your opinion about the images in attachment.
The images are from different Runs, but this is the 5th Run that we detected strange things like this bubble.
I...
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Forum: Sample Prep / Library Generation
07-12-2014, 06:52 AM
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Replies: 15
Views: 13,578
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Forum: Sample Prep / Library Generation
07-11-2014, 03:31 PM
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Replies: 15
Views: 13,578
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