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Search: Posts Made By: ShyN
Forum: Illumina/Solexa 04-23-2019, 08:36 AM
Replies: 13
Views: 2,347
Posted By ShyN
Smile We had some more issues with PF and they were...

We had some more issues with PF and they were corrected with the loading concentration being changed (raised or lowered depending on the % occupancy). We readily have 70% to 80% PF and the %Q30 have...
Forum: Core Facilities 03-25-2019, 04:12 PM
Replies: 19
Views: 15,720
Posted By ShyN
I know this is an old thread however - update! ...

I know this is an old thread however - update!

We've had pretty bad luck with the EP Motion - we use it for Illumina TruSeq library prep and we had anywhere from 40%-80% rRNA contamination when...
Forum: Illumina/Solexa 03-21-2019, 04:24 PM
Replies: 13
Views: 2,347
Posted By ShyN
Hi, can you tell me where you found this data?...

Hi, can you tell me where you found this data? Thanks!
Forum: Illumina/Solexa 03-21-2019, 08:05 AM
Replies: 13
Views: 2,347
Posted By ShyN
Smile We're going to try to so BaseSpace now - we used...

We're going to try to so BaseSpace now - we used a local application before but I think its time we give base space a chance.

We ended up concluding that our loading concentration was wayyy too...
Forum: Illumina/Solexa 03-13-2019, 02:56 PM
Replies: 13
Views: 2,347
Posted By ShyN
Hi there - yes! We have a couple used cartridges...

Hi there - yes! We have a couple used cartridges that we give you. I've sent you an email via SeqAnswers.
Forum: Illumina/Solexa 02-06-2019, 01:54 PM
Replies: 13
Views: 2,347
Posted By ShyN
For some reason, we can't see the reported...

For some reason, we can't see the reported occupancy on SAV for iSeq but we can for NovaSeq
Forum: Illumina/Solexa 02-05-2019, 04:06 PM
Replies: 13
Views: 2,347
Posted By ShyN
Exclamation iSeq, High Q30% but Low PF%

So there's not a lot of data for iSeq since its pretty new - but if anyone has had this issue on MiSeq or in general sequencing please help!

We did an iSeq run today and the results had high Q30%...
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