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Forum: RNA Sequencing 03-27-2019, 05:20 AM
Replies: 0
Views: 1,027
Posted By BioLion
StringTie merge- can someone help me understand its output?

Hello,

I am currently using StringTie merge on a StringTie output in order to discover genes that are not already in my reference annotation. I tried looking at the documentation but it is not...
Forum: Bioinformatics 11-14-2017, 04:42 AM
Replies: 0
Views: 1,160
Posted By BioLion
GAGE enrichment GO terms, KEGG pathways

Hello,
I am currently trying to find the enriched pathways following a RNAseq analysis using DESeq2, in a plant species where there is no reference genome. However, it does not work as I expected. ...
Forum: Bioinformatics 04-07-2014, 06:11 AM
Replies: 6
Views: 5,944
Posted By BioLion
Thanks again for your answers. I do realize that...

Thanks again for your answers. I do realize that this is not the ideal situation.
I'll try and contact the authors of voom/limma and then try to use the blind method if I don't find another...
Forum: Bioinformatics 04-07-2014, 05:00 AM
Replies: 6
Views: 5,944
Posted By BioLion
Thanks a lot for your clear answers. I get what...

Thanks a lot for your clear answers. I get what is the design matric now.
True, I don't have replicates, it is an exploratory analysis. Then, if I understand well, the programm wasn't able to...
Forum: Bioinformatics 04-07-2014, 01:11 AM
Replies: 6
Views: 5,944
Posted By BioLion
Limma/voom for RNA-seq data

Hello,
I am a beginner in using limma in order to analyse data and have some problems when trying to use it for the analysis of RNA-seq data.
To be more precize, I have a time course experiment,...
Forum: Introductions 04-06-2014, 09:29 PM
Replies: 0
Views: 523
Posted By BioLion
Hello!

Hello SeqAnswers,

I'm a master 2 student in bioinformatics with a background in agronomy and biotechnology, currently doing a training in Saclay, France, to analyse RNA-seq data from maize and to...
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