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Forum: Illumina/Solexa 01-08-2014, 11:56 AM
Replies: 12
Views: 7,564
Posted By haroldnunez
Is way off to A and T. I should repeat it,...

Is way off to A and T. I should repeat it, however it is wise tu use the same library?

Could PhiX be the problem as stated before?
Forum: Illumina/Solexa 01-08-2014, 11:16 AM
Replies: 12
Views: 7,564
Posted By haroldnunez
Bad news indeed, it was a V2 run. Some...

Bad news indeed, it was a V2 run. Some thumbnails.

2746

2747

2748

2749
Forum: Illumina/Solexa 01-08-2014, 10:58 AM
Replies: 12
Views: 7,564
Posted By haroldnunez
I will try your suggestion, however I'm worried...

I will try your suggestion, however I'm worried about the quality score. It could improve if I do the basecalling/demultiplexing with bcltofastq? or I just will recover the index?
Forum: Illumina/Solexa 01-08-2014, 10:40 AM
Replies: 12
Views: 7,564
Posted By haroldnunez
Thanks for your advice. About your comment, it...

Thanks for your advice. About your comment, it should have said no Index were identified. I have 18,096,508 reads but none is assigned to an index.

Anyone has experienced something like this...
Forum: Illumina/Solexa 01-08-2014, 10:26 AM
Replies: 12
Views: 7,564
Posted By haroldnunez
MiSeq run with low Q30 and high cluster PF

Hi all,

Could anybody give me an idea or though on the following?

I made a run with the MiSeq using the TrueSeq LT kit and 151 cycles. It was an amplicon library with 3 different DNA, everyone...
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