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Forum: Sample Prep / Library Generation 07-12-2013, 01:11 PM
Replies: 14
Views: 13,261
Posted By jwfoley
Update: It was actually the ladder. Apparently...

Update: It was actually the ladder. Apparently there's something weird about the binding chemistry of the NEB ladder, and Beckman Coulter has had this problem before. This protocol...
Forum: Sample Prep / Library Generation 07-10-2013, 07:30 AM
Replies: 14
Views: 13,261
Posted By jwfoley
I just got off the phone with Beckman-Coulter...

I just got off the phone with Beckman-Coulter tech support, who assured me that the RNAClean XP bead mix is exactly the same composition as AMPure XP, except prepared under RNase-free conditions....
Forum: Sample Prep / Library Generation 07-10-2013, 05:07 AM
Replies: 14
Views: 13,261
Posted By jwfoley
I don't want to size-select RNA at the moment; I...

I don't want to size-select RNA at the moment; I just happen to have a lot of these beads because they're required for other purposes and I assumed I could use the same ones for everything. But maybe...
Forum: Sample Prep / Library Generation 07-10-2013, 04:07 AM
Replies: 14
Views: 13,261
Posted By jwfoley
Unlikely. But in fact, this is the third time...

Unlikely. But in fact, this is the third time I've run this experiment. The first two times, I cut the mix with variable amounts of homemade bead buffer (20% w/v PEG 8000, 2.5 M NaCl, 10 mM Tris-HCl...
Forum: Sample Prep / Library Generation 07-09-2013, 06:04 PM
Replies: 14
Views: 13,261
Posted By jwfoley
Why aren't my SPRI beads doing a size-selection?

I'm thinking about using SPRI beads for size-selections in my library prep, but I thought I'd validate the bead ratio with an experiment similar to the one in this helpful blog post...
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