SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 7 of 7
Search took 0.00 seconds.
Search: Posts Made By: Marc_Jones
Forum: Oxford Nanopore 04-23-2019, 11:53 PM
Replies: 2
Views: 795
Posted By Marc_Jones
You could just add the index to the primers when...

You could just add the index to the primers when you order them.

or

SQK-RPB004 or SQK-PBK004 depending on your experiment.
Forum: RNA Sequencing 03-14-2019, 01:43 AM
Replies: 1
Views: 1,070
Posted By Marc_Jones
I would take 5mm x 5mm blocks of tissue and snap...

I would take 5mm x 5mm blocks of tissue and snap freeze them.
Macrodissect and homogenise in RLT using a hand homogeniser, or use a bead mill homogeniser.
Forum: Sample Prep / Library Generation 03-04-2019, 06:14 AM
Replies: 6
Views: 3,606
Posted By Marc_Jones
I've snap froze tissues without any loss of RNA...

I've snap froze tissues without any loss of RNA integrity. The biggest effect on RNA integrity is the time taken from sacrifice to freezing the tissue.

The main reason we froze was because wanted...
Forum: Oxford Nanopore 02-18-2019, 02:44 AM
Replies: 2
Views: 1,302
Posted By Marc_Jones
Which library prep method are you using? Have...

Which library prep method are you using? Have you read the protocol?
Forum: General 08-03-2018, 05:33 AM
Replies: 0
Views: 1,891
Posted By Marc_Jones
Biomek FXp

I have the opportunity to get a second hand BIOMEK FXp for library prep. What are peoples experience with them? Good, bad or indifferent? Shortfalls?

I haven't committed to anything and trying...
Forum: Illumina/Solexa 04-13-2018, 04:31 AM
Replies: 6
Views: 2,525
Posted By Marc_Jones
Thanks all

Thanks all
Forum: Illumina/Solexa 04-11-2018, 11:56 PM
Replies: 6
Views: 2,525
Posted By Marc_Jones
Edinburgher what multiplexing/pooling strategy do...

Edinburgher what multiplexing/pooling strategy do you use?

I would like to pool more than 24 samples...closer to 48 and use a NovaSeq but I don't think it's possible?
Showing results 1 to 7 of 7

 


All times are GMT -8. The time now is 10:45 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO