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Forum: RNA Sequencing 03-08-2013, 09:25 AM
Replies: 4
Views: 3,485
Posted By Paul Walker
paired t-test and differential gene testing

What I did was to use a 3rd party software called Gene Data Expressionist.
The GDE Genome Refiner module can be used with Tophat alignment bam or sam files. There are defined workflows for RNA-seq...
Forum: RNA Sequencing 08-05-2011, 11:57 AM
Replies: 4
Views: 3,485
Posted By Paul Walker
cuffdiff for differential gene testing

Hello,
I am using tophat, cufflinks, cuffcompare and cuffdiff to analyze 5 MZ twin pairs discordant for diabetes.
After the preliminary fastq groomer file conversion, the RNA-seq fastq paired end...
Forum: Introductions 06-23-2011, 03:46 PM
Replies: 0
Views: 1,064
Posted By Paul Walker
Paul W.

Hello,
I am trying to learn how to process RNA-seq files so, that I can look at my
biological data (also I assume, this is what almost everyone else is doing too).
Initially, I have a 10 sample...
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